Fig. 1.
Phenotypes of three pig breeds. A Photos of Yorkshire pigs (Y), Yorkshire × Jinhua pigs (YJ) and Jinhua pigs (J). B Body weights in Y, YJ and J at 180 days. Error bars, means ± S.D., n = 4. C Body fat ratio, average back fat thickness, intramuscular fat ratio in Y, YJ and J at 180 days of age. Error bars, means ± S.D., n = 4. D HPLC–MS/MS quantification of m5C/C in mRNA of backfat layer (BL) and longissimus dorsi muscle (LDM) in Y, YJ and J. Error bars, means ± S.D., n = 3. E qPCR analysis of NSUN2 expression in BL and LDM in Y, YJ and J at 180 days of age. 18S rRNA served as an internal RNA control. Error bars, means ± S.D., n = 3. F Western blotting of NSUN2 expression in BL and LDM in Y, YJ and J at 180 days of age. ACTB was used as a loading control. Error bars, means ± S.D., n = 3. The P values were determined using one-way ANOVA. *P < 0.05 **P < 0.01, ***P < 0.001