Fig. 4.
m5C inhibited adipogenesis in PFs and promoted myogenesis in SCs. A Western blot analysis in porcine subcutaneous fat preadipocytes (PFs) transfected with shCTL and shNSUN2 adenovirus. ACTB was used as a protein loading control. The relative protein expression was quantified by densitometry and normalized to ACTB. All data are shown as means ± S.D. B Oil Red O staining analysis in porcine subcutaneous fat preadipocytes (PFs) transfected with shCTL and shNSUN2 adenovirus. Scale bar: 100 μm. C qRT-PCR analysis in porcine subcutaneous fat preadipocytes (PFs) transfected with shCTL and shNSUN2 adenovirus. 18S rRNA served as an internal RNA control. Error bars represent means ± SD; n = 3. D Western blotting analysis in PFs transfected with control (Vector), NSUN2-WT and NSUN2-MUT adenovirus. ACTB was used as a protein loading control. The relative protein expression was quantified by densitometry and normalized to ACTB. All data are shown as means ± S.D. E Oil Red O staining analysis in PFs transfected with control (Vector), NSUN2-WT and NSUN2-MUT adenovirus. Scale bar: 100 μm. F qRT-PCR analysis in PFs transfected with control (Vector), NSUN2-WT and NSUN2-MUT adenovirus. 18S rRNA served as an internal RNA control. Error bars represent means ± SD; n = 3. G Western blotting analysis in porcine muscle satellite cells (SCs) transfected with shCTL or shNSUN2. ACTB was used as a protein loading control. The relative protein expression was quantified by densitometry and normalized to ACTB. All data are shown as means ± S.D. H Phase images analysis in porcine muscle satellite cells (SCs) transfected with shCTL or shNSUN2. Scale bar: 100 μm. I qRT-PCR analysis in porcine muscle satellite cells (SCs) transfected with shCTL or shNSUN2. 18S rRNA served as an internal RNA control. Error bars represent means ± SD; n = 3. J Western blotting analysis in SCs transfected with control (Vector), NSUN2-WT and NSUN2-MUT adenovirus. ACTB was used as a protein loading control. The relative protein expression was quantified by densitometry and normalized to ACTB. All data are shown as means ± S.D. K Phase images analysis in SCs transfected with control (Vector), NSUN2-WT and NSUN2-MUT adenovirus. Scale bar: 100 μm. L qRT-PCR analysis in SCs transfected with control (Vector), NSUN2-WT and NSUN2-MUT adenovirus. 18S rRNA served as an internal RNA control. Error bars represent means ± SD; n = 3. The p values were determined using Student’s t tests. *P < 0.05 **P < 0.01, ***P < 0.001