Fig. 7.
The role of YBX2 and SMO was mediated by ALYREF in a m5C-dependent manner. A RIP-qPCR analysis of YBX2 in PFs (left) and SCs (right) transfected with vector or ALYREF-Flag adenovirus. Error bars, means ± S.D., n = 3. B qPCR analysis of YBX2 and SMO mRNA in the nucleus or cytoplasm of control and ALYREF knockdown PF and SCs respectively. 18S rRNA served as an internal RNA control. Error bars represent means ± SD; n = 3. C Western blotting analysis in control or NSUN2-WT overexpressing PFs transfected with or without shALYREF adenovirus. ACTB was used as a protein loading control. The relative protein expression was quantified by densitometry and normalized to ACTB. All data are shown as means ± S.D. D Oil Red O staining analysis in control or NSUN2-WT overexpressing PFs transfected with or without shALYREF adenovirus. Scale bar: 100 μm. E qPCR analysis in control or NSUN2-WT overexpressing PFs transfected with or without shALYREF adenovirus. 18S rRNA served as an internal RNA control. Error bars represent means ± SD; n = 3. F Western blotting in control or NSUN2-WT overexpressing SCs transfected with or without shALYREF adenovirus. ACTB was used as a protein loading control. The relative protein expression was quantified by densitometry and normalized to ACTB. All data are shown as means ± S.D. G Phase image in control or NSUN2-WT overexpressing SCs transfected with or without shALYREF adenovirus. Scale bar: 100 μm. The P values were determined using Student’s t tests. *P < 0.05 **P < 0.01, ***P < 0.001