Fig. 2.

Vessel pruning in the mouse retinas. a Fluorescence immunohistochemistry of the mouse retinas using isolectin B4 (IB4, grey) to show the vasculature. The process of angiogenesis and vessel pruning in the mouse retina from P6 to P18. The vessel density reaches a pink at P10. Vessel pruning contributes to decreasing the vessel density from P10 to P18. Scale bar 200 μm. b Fluorescence immunohistochemistry of the mouse retinas using anti-collagen type IV (Col. IV, green) and IB4 (red) to visualize vessel pruning at P10. Arrowheads indicate the segments undergoing vessel pruning, characterized by IB4-/Col. IV + antibody staining. Scale bar 200 μm