Fig. 1.

MTSS1 was upregulated during osteoblast differentiation and downregulated in ovariectomized and aged mice. MTSS1 mRNA expression was examined in various tissues of mice using qRT-PCR. The level of MTSS1 in colon was set at 1 (A). MTSS1 expression was examined using qRT-PCR in ST2 cells (B) and primary BMSCs (C) at indicated time points during osteoblast differentiation. MTSS1 expression was examined in the BMSCs of ovariectomized mice (D) or in the calvaria of 18-month-old mice (E). Values represent mean ± SD, n = 3. One-way ANOVA was conducted in (B, C) followed by Tukey test for post hoc comparisons. Student’s t test was conducted in (D, E). *Significant vs. day 0 (B, C), sham (D) or 3-month-old mice (E), p < 0.05