Fig. 4.

MTSS1 inactivated Src and activated Wnt/β-catenin signaling in stromal progenitor cells. ST2 cells were transfected with MTSS1 expression construct (A, D) or MTSS1 siRNA (B, E) or Src expression construct (F) and their control. The protein levels of MTSS1, phosphorylated, and total Src were detected in the cells 3 days after transfection using Western blotting (A, B). Co-IP was performed and endogenous MTSS1 and LRP6 were detected with Western blotting (C). The protein levels of the major components of Wnt/β-catenin signaling were detected in the cells 3 days after transfection using Western blotting (D–F). Values represent mean ± SD, n = 3. Student’s t test was conducted in (A, D, F). One-way ANOVA was conducted in (B, E) followed by Tukey test for post hoc comparisons. *Significant vs. vector or control siRNA, p < 0.05