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. 2022 Jan 30;79(2):107. doi: 10.1007/s00018-022-04147-y

Fig. 6.

Fig. 6

Src relieved the stimulation of osteogenic differentiation induced by MTSS1. ST2 cells were cotransfected with MTSS1 and Src expression construct or the vector, and were induced to allow osteogenic differentiation (AC). Alongside, ST2 cells were transfected with control or MTSS1 siRNA, then induced to allow osteogenic differentiation in the osteogenic medium supplemented with 10 μm Src inhibitor 1 (Src-I1) (DF). ALP staining was performed in differentiated osteoblasts 14 days after osteogenic treatment (A, D). The mRNA (B, E) and protein (C, F) levels of osteogenic factors were measured 72 h after osteogenic treatment. Values represent mean ± SD, n = 3. Two-way ANOVA was conducted followed by Tukey test for post hoc comparisons. *p < 0.05