Fig. 1.

Overexpression of CRNDE hinders the progression of post-traumatic osteoarthritis in rats. An OA rat model was established using anterior cruciate ligament transectectomy. Ad-CRNDE (30 μL) or adenovirus empty vector (30 μL) were injected into the knee of OA rat. Six weeks after the operation. A, B The expression of CRNDE in knee cartilage tissues and synovial tissues was detected using qPCR. C H&E staining (Bar = 1 cm) and Safranin-O-fast green staining (Bar = 1 cm) were performed to analyze the pathological changes of the medial tibial and medial femur plateau joints in rats. And H&E staining (Bar = 1 cm) was used to assess inflammation of synovial tissues at the margin of articular cartilage. D, E Osteoarthritis Research Society International (OARSI) scores and synovitis scores. F The expression of Collagen 2 and Aggrecan in knee articular cartilage was detected with immunohistochemistry (Bar = 1 cm). G, H TUNEL staining (Bar = 1 cm) of the medial tibial and medial femur plateau joints was conducted to assess cell apoptosis, and the apoptotic cells and total cells were counted using Image J software. The sample size was n = 8, and each test was independently repeated at least three times. One-way ANOVA was used to detect differences among groups. *P < 0.05, **P < 0.01