Fig. 2.

Protective effects of CRNDE on IL-1β-induced apoptosis, inflammation and ECM degradation in chondrocyte‑like ATDC5 cells. Ad-CRNDE was used to infect chondrocyte‑like ATDC5 cells for 24 h, and then IL-1β (10 ng/mL) was used to treat cells for 24 h. A CRNDE expression was measured with qPCR. B Cell proliferation was detected using MTT assay. C Fluorescence activated cell sorting (FACS) was performed to analyze cell apoptosis. D The concentrations of IL-6, IL-8 and TNF-α in cell supernatant were determined by ELISA. E The protein levels of MMP13, ADAMTS-5, Collagen 2 and Aggrecan were measured using Western blotting. The sample size was n = 5, and each test was independently repeated at least three times. One-way ANOVA was used in A and C–E, and two-way ANOVA was used in B. *P < 0.05, **P < 0.01