Fig. 6.

CRNDE mediated H3K27 acetylation at DACT1 promoter region by binding to p300. A, B Ad-p300, sh-p300 or their respective controls were transfected into chondrocyte‑like ATDC5 cells, and the mRNA and protein levels of DACT1 were detected. Ad-p300 was normalized to Vector and sh-p300 was normalized to sh-NC. C ChIP assay showed the enrichment of p300 in the DACT1 promoter region. D RIP assay was performed to determine the binding between CRNDE and p300. E After IL-1β treatment, RIP assay was used to measure the binding of CRNDE and p300 in chondrocyte‑like ATDC5 cells. F, G After overexpression or interference with p300 in chondrocyte‑like ATDC5 cells, the enrichment of H3K27ac in DACT1 promoter was analyzed using ChIP assay. H, I After overexpression or interference with CRNDE in chondrocyte‑like ATDC5 cells, the enrichment of p300 in DACT1 promoter was assessed. J, K The mRNA and protein levels of p300 were determined after overexpressing or inhibiting CRNDE in chondrocyte‑like ATDC5 cells. Ad-CRNDE was normalized to Vector and sh-CRNDE was normalized to sh-NC. The sample size was n = 5, and each test was independently repeated at least three times. Student’s t test was used to detect differences between two groups. *P < 0.05, **P < 0.01