Fig. 3.

PTEN-null mice show hepatic FASN and O-GlcNAcylation increase levels and enhanced mTOR activation. a Livers from hepatic PTEN mutants versus wild type mice were analyzed by Western blot with indicated antibodies. Molecular mass markers are indicated on the left (kDa). b Optical densities corresponding to FASN, OGT, O-GlcNAcylation, SREBP (precursor and nuclear form), P-p70S6K, and p70S6K were measured and accordingly FASN/actin, OGT/actin, O-GlcNAc/actin, SREBP (precursor or nuclear form)/actin, and P-p70S6K/p70S6K ratios were calculated and reported. c The same samples were also analyzed by immunohistochemistry for expression of FASN, activation of mTOR pathway (phospho-rpS6) and evaluation of O-GlcNAcylation levels. d Quantification was done with Fiji software and values were accordingly represented as histograms. NS non-significant; *P < 0.05; **P < 0.01; ***P < 0.001 (n = 5)