Table 2.

Genetic reporter line in human cells

Authors	Year	References	Species	Targeted cell line	Cassette	Target region	Knock-in method	NGS platform
Choi et al	2016	[59]	Human	H9	MSGN1::EGFP	Stop codon	CRISPR/Cas9	N/A
Wu et al	2016	[94]	Human	293T, hiPSCs	PAX7::GFP	Stop codon	CRISPR/Cas9	N/A
Wu et al	2016	[96]	Human	293T, hiPSCs	MYF5::GFP	Stop codon	CRISPR/Cas9	N/A
Wu et al	2018	[91]	Human	H9/H1	MYF5::EGFP;PAX7::tdTomato	Stop codon; Stop codon	CRISPR/Cas9; CRISPR/Cas9	RNA-Seq
Choi et al	2020	[60]	Human	H9, H9, H9, H9, hiPSCs	OCT4::EGFP; MSGN1::EGFP; PAX7::EGFFP; MYOG::EGFP; RUNX1::EGFP	Stop codon; ;Stop codon; Stop codon; Stop codon; Stop codon	TALENs; CRISPR/Cas9; CRISPR/Cas9; CRISPR/Cas9; ZFN	RNA-Seq; RNA-Seq; RNA-Seq; RNA-Seq; N/A
Diaz-Cuadros et al	2020	[93]	Human	hiPSCs, hiPSCs, hiPSCs	HES7::Achilles; HES7::Achilles;pCAG::H2B::mCherry; HES7::Achilles;MESP2::mCherry	Stop codon; Stop codon; Stop codon	CRISPR/Cas9; CRISPR/Cas9; CRISPR/Cas9	Single-cell RNA-Seq; Single-cell RNA-Seq; Single-cell RNA-Seq
Tanoury et al	2020	[95]	Human	hiPSCs, hiPSCs	PAX7::Venus; MYOG::Venus	Start codon; Stop codon	CRISPR/Cas9; CRISPR/Cas9	Single-cell RNA-Seq; Single-cell RNA-Seq

N/A not available