Fig. 11.

NLRP3 inflammasome activation in BD-patient derived monocytes. Protein levels of NLRP3 (A) and pro-IL-1β (B) were quantified by WB in total extracts obtained from monocytes derived from healthy controls or BD patients under basal conditions. β-Tubulin I was used as a protein loading control to normalize the levels of the protein of interest. Data represent the mean ± SEM of results obtained in samples from 3–5 participants. Statistical significance between controls and BD patients was determined by Student’s t-test. NLRP3 protein levels were also evaluated by WB in TM-stressed monocytes derived from BD patients and healthy controls (C). β-Tubulin I was used as a protein loading control to normalize the levels of the protein of interest. Results were calculated relatively to respective basal levels and represent the mean ± SEM of results obtained in samples from 3–5 participants. Statistical differences between basal and TM-treated cells within each experimental group, and between the two groups were obtained using the two-way ANOVA test, followed by the Tukey’s post hoc test: (*p < 0.05) and (**p < 0.01). IL-1β levels (D) in supernatants from monocytes derived from healthy controls and BD patients treated with 10 μg/mL TM for 32 h, were quantified using an ELISA kit. Data represent the mean ± SEM of results obtained in samples from 5 participants. Statistical significance between controls and BD patients was determined by Student’s t-test: *p < 0.05