Fig. 2.

Stress response mediators under ER stress conditions in human THP-1 monocytes. Protein levels of PDI (A), ERO1α (B) and sigma-1R (C) were quantified by WB in total cellular extracts obtained after treatment of THP-1 cells with 5 or 10 μg/mL tunicamycin (TM), during the indicated time periods (1–24 h). β-Tubulin I was used as a control for protein loading and to normalize the levels of the protein of interest. Results were calculated relatively to control values and represent the mean ± SEM of at least three independent experiments. Statistical significance between control and TM-treated cells was determined using the one-way ANOVA test, followed by Dunnett’s post hoc test: *p < 0.05, **p < 0.01