Fig. 5.

Mitochondrial dynamics in human THP-1 monocytes upon ER stress. Protein levels of Mfn2 (A) and p-DRP1 (B) were quantified by WB in total cellular extracts obtained after incubation of THP-1 cells with 5 or 10 μg/mL TM, during the indicated time periods (1–24 h). β-Tubulin I was used as a control for protein loading and to normalize the levels of the protein of interest. Total DRP1 was used to normalize p-DRP1 levels. The membrane incubated with Sigma-1R antibody was re-probed with Mfn2 antibody and finally, re-probed with the anti-β-Tubulin I antibody. The membrane incubated with p-DRP1 antibody was then re-probed with total DRP1 antibody. Results were calculated relatively to control values and represent the mean ± SEM of at least three independent experiments. Statistical significance between control and TM-treated cells was determined using the one-way ANOVA test, followed by Dunnett’s post hoc test: *p < 0.05, **p < 0.01