Fig. 6.

Redox status under stress conditions in human THP-1 monocytes. Activity of the ROS-sensitive aconitase (A) was evaluated in total cell lysates obtained after incubation of THP-1 cells with 5 or 10 μg/mL TM for 8 h. Results were expressed as U/mg/min and represented as mean ± SEM of three independent experiments. Statistical significance of differences between control and treated cells was determined using the one-way ANOVA test, followed by Dunnett’s post hoc test. The activity of cytosolic SOD1 and mitochondrial SOD2 (B) was analyzed by using a commercial kit in total lysates obtained from cells treated with 5 or 10 µg/mL TM for 4 h. Results were normalized to untreated cells and represented as mean ± SEM of three independent experiments. Statistical differences between control and TM-treated cells were obtained with the two-way ANOVA test, followed by Sidak’s post hoc test: SOD1 (*p < 0.05) and SOD2 (^#p < 0.05) activities