Fig.7.

Mitochondrial calcium uptake and mitochondrial membrane potential under ER stress conditions in human THP-1 monocytes. Ca²⁺ influx into mitochondria was measured in THP-1 cells treated with 5 or 10 µg/mL TM for 4 or 8 h with the fluorescent probe Rhod-2/AM, before and after thapsigargin-induced ER Ca²⁺ depletion. Results were expressed by the difference between the highest post-thapsigargin value and the mean of baseline levels (A). Alterations in mitochondrial membrane potential (B) were measured using the TMRE fluorescent probe in THP-1 monocytes treated with 5 or 10 µg/mL TM for 4 or 8 h. Results were calculated relatively to control values and represent the mean ± SEM of three independent experiments. Statistical differences between control and TM-treated cells were obtained with the one-way ANOVA test, followed by the Dunnett’s post hoc test: *p < 0.05; **p < 0.01