Fig. 2.

L8 induces MT attachment and clustering of Cin8 via direct binding to MTs. A Binding of full-length Cin8 variants to surface-bound MTs, in the presence of 1 mM ATP and 110 mM KCl. Top: Representative images of MT-bound Cin8 variants; bottom: corresponding intensity profile of the GFP signal. Bar: 2 µm. B Average number of MT-bound motors per MT length, calculated on 16 fields of 3844 µm² (± SEM) at 110 mM KCl. ***P < 0.001, calculated by one-way ANOVA, followed by Tukey's test. C Intensity distribution histograms of MT-bound Cin8 variants in the assay described in (A). Maximal possible intensity of a single Cin8 monomer is indicated by black arrows (Fig. S3). D Representative SDS-PAGE profile of co-pelleting of recombinant L8 variants with MTs; L8 (aa 255–353) top panel and extended L8 (aa 251–356) bottom panel. SDS-PAGE stained with CBB. Supernatant (S) and pellet (P) of each reaction mixture is indicated on the top of each lane; Mw marker is shown on the left; Arrows indicate the sizes of the recombinant L8. Panels are representative of three experiments