Fig. 4.

L8 of Cin8 induces bi-directional motility in a multi-motor gliding assay. A Schematic representation of the multi-motor MT-gliding assay. Top panel: bacterially expressed dimeric chimera proteins, containing the biotin-binding BCCP subunit. Bottom panel: motors adsorbed onto a glass surface via avidin–biotin interaction, followed by addition of polarity-marked MTs and ATP (see Materials and Methods). B and C Representative kymographs of MT gliding driven by the different Cin8 variants at high (B) and low (C) motor densities. Bright seed indicates the minus-end of MTs. When visible, MT polarities are indicated at the bottom