Table 1.

Motile properties of WT and L8-deleted variants of Cin8

Variant	Single-molecule motility assay a,b		Multi-motor MT-gliding assay
			High-motor density d				Low-motor density h
	V (nm/s) c		Low KCl e		High KCl e
	Single molecules	Clusters	V a (nm/s)	Net displacement (%)f	V a (nm/s)	Net displacement (%)f	V a (nm/s)	Net displacement (%)f	Bi-directional motility (%)
WT CiIn8	− 15.0 ± 0.3 (41)	− 8.5 ± 0.1 (47)	30 ± 3 (94) g	29 (177)	30 ± 2 (114) g	50 (389)	n.d. i	18 (159)	45
Cin8ΔL8	− 102 ± 7 (37)	− 7.0 ± 0.2 (12)	24 ± 2 (130) g	81 (210)	24 ± 2 (126) g	56 (384)	26 ± 4 (13) g	32 (59)	0
Cin8ΔL8-D254A	− 243 ± 9 (53)	− 43 ± 3 (9)	38 ± 2*** (132) g	75 (284)	36 ± 2*** (122) g	87 (219)	38 ± 5 (38) g	56 (91)	0

^aAverages ± SEM are presented. Values in parentheses represent the number of trajectories in the single-molecule assay, or number of MTs that exhibited a net displacement ≥ 0.37 µm

^bConditions: 110 mM KCl, 1 mM ATP

^cVelocities evaluated from MD analysis; negative values represent minus-end directed motility

^dSee Materials and methods for motor concentrations

^eSee Materials and methods for KCl concentrations

^f% of MTs that exhibited a net displacement of ≥ 0.37 µm. Numbers in parentheses represent the total number of MTs in a field of 2460 µm²

^g100% of the movements were plus-end directed

^hSee Materials and methods for motor and KCl concentrations

ⁱNot determined due to low number (only ten) of polarity-labeled MTs with displacement ≥ 0.37 µm, out of which seven were plus-end directed

^***P < 0.001 compared to Cin8ΔL8, calculated using ANOVA, followed by Tukey's test