Fig. 4.

RNA pull-down study was performed with mTECs cell lysates. a Silver staining of proteins bound to Arid2-IR sense or antisense biotin-labeled probe in control or IL-1β (10 ng/mL) treated mTECs at 30 min. AS antisense. b Venn diagrams demonstrate the number of Arid2-IR binding proteins detected by Mass spectrometry in both control or IL-1β treated mTECs. c KEGG analysis show that potential Arid2-IR binding proteins especially clustered in ‘splicesome’ and ‘regulation of actin cytoskeleton’ after IL-1β treatment when compared to control group. d Flna but not NLRC5 bound with Arid2-IR confirmed by Western blot after RNA pull-down. e Arid2-IR bound with Flna compare to IgG as shown by RNA immunoprecipitation followed by qPCR. Data were shown as the relative level of Arid2-IR to β-actin. All data are mean ± SEM of at least three independent experiments. ***p < 0.001 versus IgG