Fig. 3.

Effects of dietary Fe levels on oxidative stress and the expression of HIF1α and PHD2 in the intestine tissues of yellow catfish. A Activity of antioxidant enzymes. B MDA content. C The ratio of GSH/GSSG. D The relative mRNA expression of genes involved in antioxidant. E Western blot analysis of PHD2 and HIF1α protein expression. F Quantification of PHD2, HIF1α and HIF2α relative protein level. G mRNA expression of phd2, hif1α and hif2α. H Immunofluorescent analysis of HIF1α stained with anti-HIF1α antibody (red) or DAPI nuclear stain (blue). Bars represent 250 μm. Values are shown as means ± SEM (n = 3). Bars that share different letters within the same gene indicate significant differences among the three groups (P < 0.05)