Fig. 6.

Oxidative stress mediated Fe-induced up-regulation of HIF1α protein in the primary IECs of yellow catfish. The primary IECs of yellow catfish were incubated in the control or Fe (300 μM) for 24 h in DMEM medium, or combined with 2-h MT (15 μM) pretreatment. A The confocal microscopy image of ROS generation. B, C The ROS was measured by DCFH-DA fluorescence staining. D, E The mitochondria ROS was measured by Mito-SOX fluorescence staining. F The confocal microscopy image of mitochondria ROS generation. G MDA content. H Activity of total-SOD, CAT and GPX. I The relative mRNA expression of genes involved in antioxidant responses. J, K Western blot and protein level of PHD2 and HIF1α. Values are shown as means ± SEM (n = 3). P value was calculated by Student’s t test. *P < 0.05