Fig. 8.

IGF-1 blockade in M2 macrophages suppresses the proliferation and migration of lung cancer cells. A–E After induction, M2 macrophages were cultured in RPMI 1640 medium for 48 h. Then, CM was collected for subsequent experiments. A A549 and HCC827 cells were cultured in normal medium, or macrophage CM supplemented with 5 μg/mL isotype control or 5 μg/mL IGF-1 neutralizing antibody. The proliferation of A549 and HCC827 cells was assessed by MTT assay at the indicated times. N = 5. B The survival and proliferation of A549 and HCC827 cells were determined by colony formation assay after 14 days of culture. N = 5. C Statistical analysis of the colony numbers in C. D The migration of A549 and HCC827 cells was measured by Transwell assay after 15 h of culture. N = 5. E Statistical analysis of the migrated cells in E. A, C, E The results are representative of three independent experiments. A, C, E Error bars represent the mean ± SD. p values were determined by one-way analysis of variance (ANOVA) (C, E) or two-way ANOVA (A) followed by Tukey’s post hoc test. ***p < 0.001