Fig. 2.
Food deprivation-induced activation of PVHCRFneurons is impaired in GHSR-deficient mice. A Quantitative analysis of the mean intensity of CRF + signal in the PVH of WT and GHSR-deficient mice in each experimental group. Two-way ANOVA detected condition x genotype interaction: P = 0.0004, F (1,16) = 19.44. B Percentage of CRF + cells positive for c-Fos in the PVH of WT and GHSR-deficient mice in each experimental group. Two-way ANOVA detected significant condition x genotype interaction: P = 0.0002, F (1,16) = 22.97. C Plasma corticosterone levels of WT and GHSR-deficient mice in each experimental group. Two-way ANOVA detected significant condition x genotype interaction: P = 0.0030, F (1,28) = 10.60. D Plasma glucose levels of WT and GHSR-deficient mice in each experimental group. Two-way ANOVA revealed no condition x genotype interaction, but a main effect of both condition: P < 0.0001, F (1,28) = 71.22 and genotype: P = 0.0276, F (1,28) = 5.398. Bars indicate mean ± SEM. Circles represent individual values. Tukey's multiple comparisons test shows significant differences between ‘condition’ and ‘genotype’. *p < 0.05, ***p < 0.001 and ns not significant