Fig. 4.
Food deprivation-induced activation of PVHCRF neurons requires the integrity of the ARH. A Quantitative analysis of the mean intensity of the CRF + signal in the PVH of ARH-intact and ARH-ablated mice in each experimental group. Two-way ANOVA detected significant condition x group interaction: P = 0.0012, F (1,16) = 15.39. B Percentage of CRF + cells positive for c-Fos in the PVH of ARH-intact and ARH-ablated mice in each experimental group. Two-way ANOVA detected significant condition x group interaction: P = 0.0002, F (1,16) = 23.22. C Plasma corticosterone levels of ARH-intact and ARH-ablated mice in each experimental group. Two-way ANOVA revealed no condition x group interaction, but a main effect of both condition: P < 0.0001, F (1,25) = 83.65 and group: P < 0.0001, F (1,25) = 22.26. D Plasma glucose levels of ARH-intact and ARH-ablated mice in each experimental group. Two-way ANOVA revealed no condition x group interaction, but a main effect of condition: P < 0.0001, F (1, 25) = 82.00. Bars indicate mean ± SEM. Circles represent individual values. Tukey’s multiple comparisons test shows significant differences between ‘condition’ and ‘group’. **p < 0.001, ***p < 0.001 and ns not significant