Fig. 4.

Early embryonic neurogenesis treatments with P7C3-A20 improved deficit appearance in adult SOX11-deficient mice. A and B Analysis of PROX1 ⁺ granule cells in the Sox11 cHET mouse hippocampus treated with or without P7C3-A20 intervention in the adult (8–12 W), adolescence (P21–P49), or embryonic (E12.5-P0) stage (n = 4 mice; *p < 0.01, one-way ANOVA). Scale bar, 100 μm. Error bars, mean ± SEM. C PPI of SOX11-deficient mice treated with or without P7C3-A20 intervention during the embryonic stage (n = 13; *p < 0.01, one-way ANOVA). D Hippocampus-dependent memory test in the SOX11-deficient mice treated with P7C3-A20 intervention during the adult, adolescence or embryonic stage (n = 13; *p < 0.05, Student’s t test). E Elevated Plus maze test. Representative heat maps and quantification of the cumulative movement of the SOX11-deficient mouse after treatment with P7C3-A20 intervention during embryonic neurogenesis. Error bars, mean ± SEM, n = 13, *p < 0.05, (one-way ANOVA). F Quantification of mossy fiber connections to the hippocampal CA3-SL region in Sox11 cHET mouse mice after treatment with P7C3-A20 intervention during the adult, adolescence or embryonic stage. Error bars, mean ± SEM, n = 4 mice, *p < 0.05, (one-way ANOVA)