Fig. 1.

miR-24-3p is abundantly expressed in adult skeletal muscle and upregulated during myoblast differentiation and muscle regeneration. A qRT-PCR analyses show that miR-24-3p is upregulated during mouse primary myoblast differentiation. miR-24-3p values were first normalized to the U6sn values, and the fold-change of miR-24-3p was determined relative to the undifferentiated myoblasts (GM). DM1, DM2, and DM3 indicates the number of days myoblasts cultured in differentiation medium (DM). B miR-24-3p is abundantly expressed in adult skeletal muscle. qRT-PCR was performed using various mouse tissues to measure miR-24-3p. The values were normalized to the respective U6sn values and again to the values of skeletal muscle. C Hematoxylin and eosin (H&E) staining of tibialis anterior (TA) muscle cross-sections from noninjected control and on days 1, 3, 5, 7, and 14 post-injury caused by intramuscular injection of CTX. D miR-24-3p is downregulated on days 1–3 post-injury and upregulated on days 5–14 post-injury. The values are expressed as mean ± SD of three (A–B) and five (D) biological replicates