Fig. 12.

Effect of lipoprotein particles containing WT apoA-I or apoA-I[A164S] on ROS formation by EA.hy926 cells. a The formation of ROS by EA.hy926 cells treated with or without rHDL containing WT apoA-I or apoA-I[A164S] for 24 h was measured following incubation of cells with DCFH-DA and detection of fluorescent DCF emitted from cells using a fluorescence microscope. Representative microscopic views are shown. b Images were scanned and quantified by Image J software. The relative DCF fluorescence is shown. Values are the means ± SD (n = 6). ^#p < 0.01; ^##p < 0.001