Fig. 8.

Effect of L144R, A164S and L178P mutations on the capacity of lipoprotein-associated apoA-I to promote the migration of endothelial cells. a EA.hy926 cells or c HCAEC were wounded and either left untreated or treated with rHDL containing WT or mutant apoA-I forms for 24 h. Representative microscopic views at 0 h and 24 h are shown. Images were scanned and quantified by ImageJ software. b Quantitative analysis of wound area closure of EA.hy926 cells after 24 h. d Number of HCAEC migrated after 24 h. Values are the means ± SD for three independent experiments performed in duplicate. ***p < 0.0001 versus apoA-I WT