Table 1.

Summary of the observed changes in biophysical and functional properties of apoA-I L144R, A164S and L178P mutants as compared to WT apoA-I

	Change in property as compared to WT apoA-I
	apoA-I[L144R]	apoA-I[A164S]	apoA-I[L178P]
Biophysical properties (lipid-free apoA-I)
Helicity (%)	~ 4 ↓	~ 4 ↓	~ 11 ↓
Thermal unfolding
App Tm (οC)	~ 2.5 ↓	~ 2.5 ↓	14.3 ↓
App ΔH (kcal/mol)	5.5 ↓	↔	15.6 ↓
Chemical unfolding
D1/2 (M)	0.1 ↓	~ 0.1 ↓	0.4 ↓
ΔGoD (kcal/mol)	↔	1.4 ↓	3.2 ↓
ANS binding	↔	↔	22% ↑
Biophysical properties (rHDL-apoA-I)
Helicity (%)	~ 8 ↓	↔	~ 6 ↓
Thermal unfolding
T1/2 (οC)	~ 3 ↓	↔	~ 3 ↓
Functional properties (lipid-free apoA-I)
ABCA1-mediated cholesterol efflux	↔	↔	~ 35% ↓
Functional properties (rHDL-apoA-I)
LCAT activation	~ 70% ↓	↔	↔
SR-BI-mediated cholesterol efflux	~ 40% ↓	↔	~ 25% ↓
ABCG1-mediated cholesterol efflux	↔	~ 60% ↓	↔
Endothelial cell migration
EA.hy926	↔	~ 50% ↓	↔
HCAEC	↔	~ 100% ↓	↔

An upward-pointing arrow indicates that the property has increased, a downward-pointing arrow indicates that the property has decreased and a left–right arrow indicates no change