Table 3.
Delivery methods for CRISPR/Cas system in plants
| Species | Delivery methods | Edited gene | References | |
|---|---|---|---|---|
| Stable | Transient | |||
| Arabidopsis thaliana | Protoplast, Agroinfiltration | PDS3, FLS2, RACK1b and RACK1c | [122] | |
| Arabidopsis thaliana | Agroinfiltration | GFP | [215] | |
| Arabidopsis thaliana | Agrobacterium | GFP | [216] | |
| Arabidopsis thaliana | Agrobacterium | Protoplast | BRI1, JAZ1, GAI, and YFP | [122] |
| Arabidopsis thaliana | Agrobacterium | BRI1, JAZ1, GAI, CHLI1/2, AP1, TT4 and GUUS | [217] | |
| Arabidopsis thaliana | Agrobacterium | Protoplast | CHLI1, CHLI2 and TT4 | [218] |
| Arabidopsis thaliana | Agrobacterium | ADH1 | [219, 220] | |
| Arabidopsis thaliana | Agrobacterium | TRY, CPC and ETC2 | [221] | |
| Arabidopsis thaliana | Agrobacterium | At5g55580 | [145] | |
| Arabidopsis thaliana | Agrobacterium | ADH1 and TT4 | [222] | |
| Arabidopsis thaliana | Agrobacterium | BRI1 | [224] | |
| Arabidopsis thaliana | Agrobacterium | ALS | [225] | |
| Arabidopsis thaliana | Agrobacterium | ETC2, TRY, CPC and CHLI1/2 | [226] | |
| Arabidopsis thaliana | Agrobacterium | FT and SPL4 | [227] | |
| Arabidopsis thaliana | Agrobacterium | AP1, TT4 and GL2 | [228] | |
| Arabidopsis thaliana | Agrobacterium | PDS3, AG, DUO1 and ADH1 | [229] | |
| Arabidopsis thaliana | Agrobacterium | At3g04220 | [230] | |
| Nicotiana benthamiana | Agrobacterium | Agroinfiltration | PDS | [121] |
| Nicotiana benthamiana | Agroinfiltration | PDS | [123, 262, 231] | |
| Nicotiana benthamiana | Virus | PCNA and PDS | [136] | |
| Nicotiana benthamiana | Agroinfiltration | GFP | [215] | |
| Nicotiana benthamiana | Virus | PDS, IspH and fsGUS | [140] | |
| Nicotiana tabacum | Protoplast | PDS and PDR6 | [232] | |
| Nicotiana tabacum | Virus | SurA and SurB | [138] | |
| Nicotiana tabacum | Protoplast | PDS | [132] | |
| Nicotiana tabacum | Agrobacterium | PDS and STF1 | [233] | |
| Nicotiana tabacum | Protoplast | AOC | [234] | |
| Populus trichocarpa | Agrobacterium | PDS | [235] | |
| Oryza sativa | Agrobacterium | ROC5, SPP and YSA | [122] | |
| Oryza sativa | Protoplast | SWEET11 and SWEET14 | [215] | |
| Oryza sativa | Agroinfiltration | PDS | [121] | |
| Oryza sativa | Biolistic | Protoplast | PDS-SP1, BADH2, Os02g23823 and MPK2 | [144] |
| Oryza sativa | Agrobacterium | Protoplast | MYB1 | [218] |
| Oryza sativa | Protoplast | MPK5 | [236] | |
| Oryza sativa | Agrobacterium | CAO1 and LAZY1 | [237] | |
| Oryza sativa | Agrobacterium | PTG1, PTG2, PTG3, PTG4, PTG5, PTG6, PTG7, PTG8 and PTG9 | [236] | |
| Oryza sativa | Agrobacterium | OsBEL | [238] | |
| Oryza sativa | Agrobacterium | FTL, GSTU, MRP15 and Waxy | [145] | |
| Oryza sativa | Agrobacterium | Protoplast | SWEET1a, SWEET1b, SWEET11, SWEET13 and CYP76 | [239] |
| Oryza sativa | Agrobacterium | PDS, PMS3, EPSPS, DERF1, MSH1, MYB5, MYB1, ROC5, SPP and YSA | [240] | |
| Oryza sativa | Agrobacterium | DMC1A | [241] | |
| Oryza sativa | Protoplast | PDS, DEP1, ROC5 and miR159b | [242] | |
| Oryza sativa | Agrobacterium | DL and ALS | [233] | |
| Oryza sativa | Protoplast | PDS | [132] | |
| Triticum aestivum | Protoplast | Mlo | [144] | |
| Triticum aestivum | Biolistic | Protoplast | Gw2 | [147] |
| Triticum aestivum | Agroinfiltration | Inox and Pds | [231] | |
| Zea mays | Protoplast | zm ipk | [128] | |
| Zea mays | Agrobacterium | Protoplast | zm hkt1 | [221] |
| Zea mays | Biolistic | lig1, ms26, ms45, als1 and als2 | [146] | |
| Zea mays | Biolistic | lig, ms26, ms45 and als2 | [148] | |
| Zea mays | Biolistic | argos8 | [243] | |
| Glycine max | Agrobacterium | GFP, Glyma07g14530, 01gDDM1, 11gDDM1, Met1-04g, Met1-06g, miR1514 and miR1509 | [244] | |
| Glycine max | Biolistic | DD20, DD43 and ALS1 | [261] | |
| Glycine max | Agrobacterium | Glyma06g14180, Glyma08g02290 and Glyma12g37050 | [246] | |
| Glycine max | Agrobacterium | bar, FEI1, FEI2 and SHR | [245] | |
| Glycine max | Agrobacterium | PDS11 and PDS18 | [247] | |
| Glycine max var | Protoplast | FAD2-1A and FAD2-1B | [234] | |
| Solanum tuberosum | Agrobacterium | StIAA2 | [223] | |
| Solanum tuberosum | Agrobacterium | StALS1 | [248] | |
| Solanum tuberosum | Protoplast | GBSS | [142] | |
| Hordeum vulgare | Agrobacterium | HvPM19 | [249] | |
| Marchantia polymorpha | Agrobacterium | ARF1 | [250] | |
| Solanum lycopersicum | Agrobacterium | SlAGO7 | [251] | |
| Solanum lycopersicum | Virus | ANT1 | [127] | |
| Solanum lycopersicum | Agrobacterium | RIN | [252] | |
| Brassica oleracea | Agrobacterium | BolC.GA4.a | [249] | |
| Papaver somniferum | Agroinfiltration | 4′OMT2 | [253] | |
| Papaver somniferum | Agrobacterium | eIF4E | [254] | |
| Xanthomonas citri subsp. citri | Agrobacterium | Agroinfiltration | PDS | [255] |
| Xanthomonas citri subsp. citri | Agrobacterium | Agroinfiltration | LOB1 | [256] |
| Vitis vinifera | Agrobacterium | IdnDH | [257] | |
| Chardonnay | Protoplast | MLO-7 | [258] | |
| Golden delicious | Protoplast | DIPM-1, DIPM-2 and DIPM-4 | [258] | |