Fig. 3.

ZBTB28 regulated IFNAR to activate ISGs. A qRT-PCR results of IFNAR1 and IFNAR2 mRNA expression in stable transfected breast cancer cells. B Structure of wild-type IFNAR1 and IFNAR2 reporter plasmid. The sequence of the predicted site has been displayed. C Promoter luciferase activity of IFNAR1 and IFNAR2 in 293 T and MCF7 cells. D ChIP analysis with HA-tag for ZBTB28 binding to the IFNAR1 or IFNAR2 promoter. Then, products of ChIP-PCR were used for electrophoresis. E, F Cancer cells were pretreatment with/without IFNAR inhibitor (1 μM) for 8 h and then co-cultured with THP-1 macrophages for another 24 h later. Expressions of ISGs in the indicated groups were detected by qRT-PCR