Fig. 6.

Over-expression of ZBTB28 promotes phagocytosis of tumor cells by macrophages. A, B Representative flow-cytometry plots portraying the phagocytosis of MB231 cells treated with anti-CD24 mAb, anti-CD47 mAb or dual treatment, compared with the IgG control. C Phagocytosis efficiency was shown as a bar graph. D Representative phagocytosis images of THP-1 macrophages engulfing MB231 cells with vector and ZBTB28 overexpression. THP-1 macrophages were stained green (Calcein-AM); cancer cells were labeling with red (DIL). The white arrows indicate macrophages that engulfed cancer cells. (E) Representative pictures showing tumor growth 14 days after subcutaneously implanted different MB231 cells. (F, G) Tumors’ volume and weight were measured, respectively (n = 4). All statistical data were shown as mean ± SEM.*p < 0.05, **p < 0.01, ***p < 0.001