Table 1.
Current methods to determine HSC quiescence
| Label | Labeling | Reference |
|---|---|---|
| Content of DNA and RNA | Hoechst + Pyronin Y | [20, 21] |
| DAPI/7AAD + BrdU | [23] | |
| DAPI/7AAD + EdU | [24, 25] | |
| Proliferative markers and content of DNA | Ki-67 + DAPI/Hoechst | [28, 29] |
| Cell surface markers | CD41 | [31] |
| CD229 + CD244 | [32] | |
| CD63 | [33] | |
| CD82 | [34] | |
| Content of nucleosomes | H2B-GFP | [36, 37] |
| Mitochondrial membrane potential | Tetramethylrhodamine ethyl ester | [37] |
| Reactive oxygen stress | DCFH-DA | [38] |
| Transcriptional dynamics | Single-cell-RNA-sequence | [39, 40] |