Fig. 5.

Significantly increased DAG content and PC synthesis-related genes were observed in gWAT of ASKO mice. a Heatmap of the extensively changed DAGs in gWAT from WT and ASKO mice (n = 6 mice/group). b The concentration of the increased DAGs is shown. c The mRNA expression levels of PC synthesis-related genes and phospholipase A2 genes in gWAT of two groups of mice (n = 6 mice/group). 18s was used as an internal control. d Western blotting analysis of CTα protein level in gWAT from 6-month-old WT and ASKO mice (n = 3 mice/group). e The relative protein level of CTα was normalized to β-Tubulin. f Schematic representation of the process of PC and LysoPC synthesis. The red words display the upregulated genes, and the blue words display the downregulated genes in gWAT of ASKO mice. g Western blotting analysis of RNF20 protein level in siNC- and siRNF20-transfected mature adipocytes. h The level of LysoPC in culture media derived from siNC and siRNF20 cells was detected by ELISA (n = 6 samples/group). Data are presented as the mean ± SEM. *P < 0.05, **P < 0.01 and ***P < 0.001