Fig. 8.

Immunotherapy targeting PAI-1 improves pathological signs of PLP-induced EAE animals. a Representative immunostaining of fibrin(ogen) (red), CD4 (yellow) and myelin (MBP, grey) in the spinal cord from control and anti-PAI-1 antibody-treated mice at acute (16 dpi), remission (28 dpi) and late stages (45 dpi) during PLP-induced EAE, (DAPI: blue). Demyelinated areas are defined by the dashed yellow line. The representative images for acute stages are from the cervical region and for remission and late stages are from the lumbar area. Corresponding quantifications of b fibrin(ogen) deposits, c CD4⁺ cells infiltration and d demyelination. Data are represented as mean ± SEM. n = 3 per condition. Mann–Whitney U test. **p < 0.01; ***p < 0.001. Scale bars: 50 µm