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Plasmids carrying rDNA which were used to support growth of rdnΔΔ strains. Structures of plasmids pRDN-hyg1, pNOY373 (p-Pol I) and pNOY353 (p-Pol II) are shown. Plasmid pRDN-hyg1 carries a T→C mutation at the site (indicated by an asterisk) encoding position 1756 of 18S rRNA (13). E in the rDNA carried by pNOY373 is the segment containing T/E/RFB (see Fig. 1) and two flanking lines indicate the two restriction sites created by mutagenesis.
