Fig. 4.

NSun2 deficiency reduces ATX upregulation in endothelium and plasma of HHcy-aggravated AAA. Infrarenal abdominal aortas of 10- to 11-week-old WT and NSun2^+/− mice were treated with saline or elastase to induce abdominal aortic aneurysm after the mice were fed a normal chow diet and given drinking water supplemented with or without 1.8 g/L Hcy for 2 weeks. a Representative Western blot analysis of ATX expression in infrarenal abdominal aortas. b Real-time PCR quantification of ATX mRNA levels in infrarenal abdominal aortas (n = 6). c Representative immunofluorescent staining of ATX expression in endothelial cells (CD31 positive) in infrarenal abdominal aortas. Scale bar, 25 μm. d Plasma ATX levels were measured via ELISA (n = 6). Data shown are mean ± SEM. *P < 0.05, by two-way ANOVA followed by Tukey’s test for multiple comparisons (b, d). ns no significance