Fig. 2.

Examples of CDR3 sequences in a single B cell that can assemble into single BCR or multiple BCR in both human (HB08) and mouse(PBMC_C57BL/6). A Pairing of heavy and light chains that can assemble into different types of single and dual (or multiple) BCR in human (HB08) sample. B Examples of CDR3 sequences and the V(D)J recombination in a single B cell of human (HB08) sample. C Pairing of Heavy and Light chains that can assemble into different types of single and dual (or multiple) BCR in mouse (PBMC_C57BL/6) sample. D Examples of CDR3 sequences and the V(D)J recombination in a single B cell of mouse (PBMC_C57BL/6) sample. The proportion of H + K pairing was significantly higher than H + L in the single BCR B cells of both human (HB08) and mouse (PBMC_C57BL/6) samples. However, the proportion of H + L pairing was much lower in mouse compared to human, dual or multiple BCR in both species are mainly H + K + K and H + K + L, indicating that Kappa is utilized more frequently than Lambda in single or dual BCR. There are cells expressing three H chains (three K chains or three L chains, etc.) in “others”, examples of the V_H(D)J_H recombination patterns and CDR3 sequences in single cells expressing different types of single and dual (or multiple) BCR B cells in both human peripheral blood (HB08) and mouse (PBMC_C57BL/6) samples