Fig. 3.

Inhibition of LAMC2 significantly decreased the growth of PDAC cells in-vitro. A, B. LAMC2 shRNA stable clones of HPAC, Panc0403, Panc0504, and Panc0327 displayed depletion of LAMC2 both at the transcript and protein levels than scramble (SCR) shRNA. GAPDH primers and antibodies were used for normalization and loading controls in qRT-PCR and western blotting experiments, respectively. Experiments were performed thrice in biological triplicates and the results represent mean ± SD. **P-value < 0.001; ***P-value < 0.0001; two-tailed t-test. C. LAMC2 shRNA displayed reduced growth of HPAC, Panc0504, and Panc0327 cells. Results represent mean ± SD; n = 3. **P-value < 0.001; ***P-value < 0.0001; two-tailed t-test. D LAMC2 depletion showed decreased clonogenic growth of HPAC, Panc0504, and Panc0327 cells. Data represent mean ± SD; n = 3. ***P-value < 0.0001;**P-value < 0.001 two-tailed t-test