Fig. 1.

Aquaporins and inflammatory markers expression and their modulation by LPS in monocytes and THP-1 cells. a Relative expression of AQPs in resting monocytes. b Relative expression of AQPs in resting THP-1 cells. c Expression of AQPs in LPS-treated monocytes normalized to non-LPS (dotted line) for each AQP. d Expression of AQPs in PMA and PMA + LPS-treated THP-1 cells normalized to non-PMA-treated cells (dotted line) for each AQP. e Expression of IL-6, TNFα, and IL-1β in LPS-treated monocytes normalized to non-LPS (dotted line). Gene expression values are relative to HPRT-1. f Representative blots and relative AQP3 protein expression in non-PMA, PMA, and PMA + LPS-treated THP-1 cells in relation to α-tubulin. g Expression of the AQP3 silencing in THP-1 cells by qPCR normalized to wild-type cells (dotted line). h Expression of AQP3 transfected THP-1 cells treated PMA or PMA + LPS. Data represent mean ± SD of three independent experiments. *P < 0.05, **P < 0.01; ***P < 0.001; *LPS vs resting cells