Fig. 2.
AQP3 is a functional glycerol channel in THP-1. a Water and glycerol permeabilities measured by stopped-flow spectroscopy in THP-1 cells. b Activation energy for water and glycerol transport in THP-1 cells. c Glycerol permeability (Pgly) in THP-1 cells before and after 10 μM Auphen treatment. d, e Time course of cell volume change after an osmotic challenge with mannitol (d) or with glycerol (e), measured in adherent THP-1 cells by fluorescence microscopy, to evaluate permeability. f, h Representative water (f) and glycerol (h) permeability measurements in untreated, PMA-treated, or PMA + LPS-treated THP-1 before and after Auphen treatment. g Water permeability (Pf) in THP-1 treated as in (f). i Glycerol permeability (Pgly) in THP-1 treated as in (h). a–c, Bars show mean ± SD from 4 independent experiments. d–i Bars show mean ± SD from 10 cells analyzed on 3 coverslips in 2 different cell platings. ##P < 0.01, ###P < 0.001; #Auphen-treated vs non-treated cells