Fig. 3.

AQP3 involvement in macrophage priming. a IL6 mRNA expression (left) and protein release (right) in PMA and PMA + LPS-treated THP-1 in the absence and presence of 10 μM Auphen or in AQP3-silenced cells. b IL1β mRNA expression in THP-1 cells treated as in (a). c Representative blots (left) and relative pro-IL1β protein expression in relation to α-tubulin (right) in THP-1 treated with PMA + LPS in the presence and absence of Auphen or in AQP3-silenced cells. d TNFα release in PMA and PMA + LPS-treated THP-1 in AQP3-silenced cells. e Percentage of extracellular LDH in cells treated as in (a). Bars show mean ± SD from 3 independent experiments. *P < 0.05; **P < 0.01; ***P < 0.001; *PMA + LPS vs PMA. ^#P < 0.05; ^##P < 0.01; ^#Auphen vs without Auphen; ^§P < 0.05; ^§§P < 0.01; ^§AQP3 shRNA vs Scramble shRNA