Fig. 4.

Swelling AQP3-dependent inflammasome activation. a IL-1β release and b percentage of cell death after imposing glycerol cell swelling in THP-1 cells non-treated and treated with 10 μM Auphen, or in AQP3 shRNA cells as indicated. Wild-type (WT), caspase-1 deficient (Casp-1 KO), and scramble shRNA THP-1 were used. c Intracellular K⁺ content of PMA + LPS-treated wild-type THP-1 cells after swelling or shrinking in the presence or absence of Auphen or in AQP3 shRNA cells as indicated. Values are normalized to control cells. Bars show mean ± SD from three independent experiments. **P < 0.01; *Swelling vs no challenge. ^#P < 0.05; ^###P < 0.001; ^#Auphen vs without Auphen; ^§P < 0.05; ^§§P < 0.01; ^§AQP3 shRNA vs scramble shRNA