Fig. 5.

Contribution of AQP3 for inflammasome activation. a, b IL-1β release measured by ELISA, and c, d percentage of cell death after challenging PMA + LPS primed THP-1 cells with nigericin (a, c) and ATP (b, d). e Intracellular K⁺ content of PMA + LPS primed THP-1 cells after nigericin stimulation in non-treated and Auphen-treated cells; values were normalized to control cells. All experiments were done in wild-type (WT) and Caspase-1 knocked-out (Casp-1 KO) cells non-treated and treated with Auphen and AQP3 shRNA cells. Bars show mean ± SD from 3 independent experiments. **P < 0.01, ***P < 0.001; *Nigericin/ATP vs no challenge. ^#P < 0.05, ^##P < 0.01; ^#Auphen vs without Auphen. ^§P < 0.05, ^§§P < 0.01; ^§AQP3 shRNA vs Scramble shRNA