Table 3. Performance of the four assemblers under evaluation for ESTs representing 73 known genes with an average coverage of 196 ± 180 sequences.

	Phrap	CAP3	TA-EST	TIGR Assembler
(A)
No. of single assemblies	46	59	15	2
Mean no. of assemblies	1.56	1.26	2.85	17.26
Standard deviation	0.88	0.58	1.79	17.20
(B)
Mean no. of singletons	0.07	0.10	8.05	38.55
Standard deviation	0.35	0.45	10.09	47.14

For each of these genes, one would expect the assembler to produce a single consensus without singletons. (A) The number of single contigs produced by each assembler and the mean and standard deviation of the number of assemblies. (B) The mean and standard deviation of the number of singletons produced by each of the assemblers.