Table 1.

List of oligonucleotides used in this study

Name	Sequence
Myc-MDH1K118R	F 5′-gggcacagccttggagAGAtacgccaagaaatcag-3′
Myc-MDH1K118R	R 5′-ctgatttcttggcgtaTCTctccaaggctgtgccc-3′
Myc-MDH1K121R	F 5′-ccttggagaaatacgccAGAaaatcagttaaggtcattgt-3′
Myc-MDH1K121R	R 5′-acaatgaccttaactgatttTCTggcgtatttctccaagg-3′
Myc-MDH1K298R	F 5′-gatcaagaataagacctggAGAtttgttgaaggcctccccat-3′
Myc-MDH1K298R	R 5′-atggggaggccttcaacaaaTCTccaggtcttattcttgatc-3′
Myc-MDH1K121Q	F 5′-ggcacagccttggagaaatacgccCAGaaatcagttaagg-3′
Myc-MDH1K121Q	R 5′-ccttaactgatttCTGggcgtatttctccaaggctgtgcc-3′
Myc-MDH1K298Q	F 5′-caagaataagacctggCAGtttgttgaaggcc-3′
Myc-MDH1K298Q	R 5′-ggccttcaacaaaCTGccaggtcttattcttg-3′
Myc-MDH13KR	F1 5′-gcacagccttggagAGAtacgccAGAaaatcagttaaggtcattgttgtggg-3′
Myc-MDH13KR	R1 5′-acaacaatgaccttaactgatttTCTggcgtaTCTctccaaggctgtgccc-3′
Myc-MDH13KR	F2 5′-gatcaagaataagacctggAGAtttgttgaaggcctccccat-3′
Myc-MDH13KR	R2 5′-atggggaggccttcaacaaaTCTccaggtcttattcttgatc-3′
Myc-MDH12KR	F1 5′-ccttggagaaatacgccAGAaaatcagttaaggtcattgt-3′
Myc-MDH12KR	R1 5′-acaatgaccttaactgatttTCTggcgtatttctccaagg-3′
Myc-MDH12KR	F2 5′-gatcaagaataagacctggAGAtttgttgaaggcctccccat-3′
Myc-MDH12KR	R2 5′-atggggaggccttcaacaaaTCTccaggtcttattcttgatc-3′
Myc-MDH12KQ	F1 5′-ggcacagccttggagaaatacgccCAGaaatcagttaagg-3′
Myc-MDH12KQ	R1 5′-ccttaactgatttctgggcgtaTTTctccaaggctgtgcc-3′
Myc-MDH12KQ	F2 5′-caagaataagacctggCAGtttgttgaaggcc-3′
Myc-MDH12KQ	R2 5′-ggccttcaacaaaCTGccaggtcttattcttg-3′
shMDH1	F 5′-ccggggatcttactgcaaaggaactctcgagagttcctttgcagtaagatcctttttg-3′
shMDH1	R 5′-attcaaaaaggatcttactgcaaaggaactctcgagagttccttggcagtaagatcc-3′

Primers used for the mutant MDH1 vectors and shRNAs against MDH1 were listed