Fig. 1.

LRP4 binds Scl with higher affinity than LRP6 and enhances Scl binding to LRP6. a Yeast surface display (YSD) affinity titration curve. Recombinant yeast cells expressing Scl were incubated with soluble LRP6 (0.01–650 nM) or LRP4 (0.1–100 nM). b Geo MFI, presented as fold change. Recombinant yeast cells expressing Scl were pre-incubated with the indicated amounts of soluble LRP4, followed by incubation with soluble LRP6-Fc (200 nM). Points represent individual Geo MFI values of three independent experiments. Significance was assessed using unpaired, two-tailed Student's t test