Fig. 3.

Scl_N93A relieves Wnt3a-activated signaling inhibition by Scl in MC3T3-E1 cells and this activity is LRP4 dependent. Wnt pathway activation in MC3T3 demonstrated by luciferase activity (measured as fold change) in a super-TOPFlash assay (a–c). Cells were treated with Wnt3a conditioned medium (CM) and a Scl_N93A (6–620 nM) or b Scl (6 nM) in the absence or presence of Scl_N93A (6–620 nM). c Cells were transiently transfected with LRP4 specific siRNA (si1), or nontargeting siRNA (NC) and different combinations of Scl and Scl_N93A. Points represent individual measurements of biological replicates (A–C, n = 9) collected in three separate experiments. Significance was assessed using unpaired, two-tailed Student's t test. An outlier identified using Grubbs' test was removed in two cases (treatment with Wnt3a CM and 124 nM Scl_N93A, and treatment with Wnt3a CM, 6 nM Scl, and 124 nM Scl_N93A)